The aSURE™ instrument fitting was developed as a means to maintain a sterile barrier where disposable manifolds are used on hybrid single-use process equipment. Fixed or tethered pressure-monitoring devices provide extremely high accuracy and are often hard-wired into a central control panel. The aSURE™ instrument fitting provides a practical means of maintaining a sterile barrier on a complex manifold set and provides a barrier without the need to have a gauge present during the sterilization process.